Journal: Cells

Article Title: Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

doi: 10.3390/cells10112998

Figure Lengend Snippet: Doxorubicin increases FGF-1 and PDGF-B protein levels in adult heart ventricular cells. Mice were given intraperitoneal injections of saline (S) or doxorubicin (D) and their hearts were harvested after 24 h (Day 1) or 72 h (Day 3). Using Western blotting, lysates containing equal amounts of total protein from the ventricular tissue of the differently treated animals were tested for FGF-1 and PDGF-B protein levels. When standardized against GAPDH, the level of both proteins (FGF-1 and PDGF-B) was found to be significantly higher in the Dox-treated ventricles than in the saline treated ventricles by Day 3. * p < 0.05 vs. saline (S), One-way ANOVA with Tukey’s multiple comparisons test, N = 3 independent experiments for each group.

Article Snippet: For the analysis of growth factor effects, the wells contained 1% FBS (Low Serum) DMEM and 40 ng/mL of FGF-1 (rMuFGF1 TM , Gold Biotechnology, St. Louis, MO, USA), its neutralizing antibody (Santa Cruz sc-7910, 100 ng/mL), or both.

Techniques: Western Blot

Journal: Cells

Article Title: Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

doi: 10.3390/cells10112998

Figure Lengend Snippet: FGF-1 and PDGF-B increase embryonic ventricular cell migration, whereas their neutralizing antibodies decrease migration. 300 μL of 0.5 × 10 6 cells/mL serum-free cell suspensions were incubated for 22 h at 37 °C in polycarbonate membrane inserts with 8 μm pores, with surrounding wells containing 1% FBS DMEM and FGF-1, its antibody, or both ( A ) or PDGF-B, its antibody, or both ( B ). The inserts were then removed, migratory cells that had passed through the pores were washed and stained, and the cell stain was solubilized for measurement of absorbance. * p < 0.05 vs. all other groups; # p < 0.05 vs. all other groups, One-way ANOVA with Tukey’s multiple comparisons test, N = 3 independent experiments for each group.

Article Snippet: For the analysis of growth factor effects, the wells contained 1% FBS (Low Serum) DMEM and 40 ng/mL of FGF-1 (rMuFGF1 TM , Gold Biotechnology, St. Louis, MO, USA), its neutralizing antibody (Santa Cruz sc-7910, 100 ng/mL), or both.

Techniques: Migration, Incubation, Staining

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: IHC staining of mp53 in SCCHN samples shows mainly nuclear and some cytoplasmic expression patterns. IHC staining of FGFR3 shows membrane and cytoplasmic expression. Four representative images are presented: mp53+/FGFR3+, mp53+/FGFR3-, mp53-/FGFR3+, mp53-/FGFR3- and negative control. (200 x magnification).

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques: Immunohistochemistry, Expressing, Membrane, Negative Control

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Univariate association of FGFR3 with clinical characteristics in cohort 1.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: FGFR3 association with clinical characteristics in cohort 2.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Correlation of FGFR3 with p53 and mp53 in cohort 1.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Pearson correlation coefficients (p-values) between FGFR3 and mp53, cytoplasmic and nuclear mp53 when p16 is negative.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Pearson correlation coefficients (p-values) between FGFR3 and mp53, cytoplasmic and nuclear mp53 when p16 is positive.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Cohort 1, univariate analysis of overall survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Cohort 1, univariate analysis of disease free survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: Multivariate survival analysis on disease free survival.

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques:

Journal: PLoS ONE

Article Title: Co-expression of fibroblast growth factor receptor 3 with mutant p53, and its association with worse outcome in oropharyngeal squamous cell carcinoma

doi: 10.1371/journal.pone.0247498

Figure Lengend Snippet: KM plots were generated using median weighted index (WI) of mp53 (3.5) and FGFR3 (35) as the cut off values (>median as positive and ≤ median as negative). High FGFR3 and mp53 levels correlated with worse OS (A) and DFS (B).

Article Snippet: Besides hematoxylin and eosin (H&E) staining, standard immunohistochemistry (IHC) was used to stain for p16 (1:100, Delta Biolab, Gilroy, CA), FGFR3 (PA2143, Boster Bio, Pleasanton, CA.) and mp53 (AB32049, Abcam, Cambridge, MA.) using primary antibodies followed by corresponding secondary antibodies, visualized by DAB and counterstained with hematoxylin.

Techniques: Generated